Publikationen

Publikationen aus unserer Forschung im Überblick

3757376 3ITDHJV5 1 apa 50 default 526 https://www.molit.eu/wp-content/plugins/zotpress/
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Larribère, L., Gordejeva, J., Kuhnhenn, L., Kurscheidt, M., Pobiruchin, M., Vladimirova, D., Martin, M., Roser, M., Schramm, W., Martens, U. M., & Eigenbrod, T. (2021). Assessment of SARS-CoV-2 Infection among Healthcare Workers of a German COVID-19 Treatment Center. International Journal of Environmental Research and Public Health, 18(13), 7057. https://doi.org/10.3390/ijerph18137057
Latzer, P., Zelba, H., Battke, F., Reinhardt, A., Shao, B., Bartsch, O., Rabsteyn, A., Harter, J., Schulze, M., Okech, T., Golf, A., Kyzirakos-Feger, C., Kayser, S., Pieper, N., Feldhahn, M., Wünsche, J., Seitz, C., Hadaschik, D., Garbe, C., … Biskup, S. (2024). A real-world observation of patients with glioblastoma treated with a personalized peptide vaccine. Nature Communications, 15(1), 6870. https://doi.org/10.1038/s41467-024-51315-8
Vladimirova, D., Bochum, S., Weiß, M., & Martens, U. M. (2022). Molekulares Profiling für Diagnose und Therapieentscheidung beim CUP Syndrom: Fallbericht aus einer monozentrischen Studie. Oncology Research and Treatment, 45(suppl 2), 5–346. https://doi.org/https://doi.org/10.1159/000526456
Vladimirova, D., Mbonu, P., Umapathy, M., Kothapalli, P., Otmacic, G., Akkad, J., Weyersberg, S., Martens, U. M., & Jechlinger, M. (2022). Etablierung einer Gewebe-Pipeline für multidimensionale Analysen beim metastasierten kolorektalen Karzinom. Oncology Research and Treatment, 45(suppl 2), 5–346. https://doi.org/https://doi.org/10.1159/000526456
Bochum, S., Ulmer, M., Rohlfing, S., Schmohl, J., Löffler, H., Knott, M., Weßendorf, S., Hänle, C., Greiner, J., Denzlinger, C., Illerhaus, G., Ganten, T., Sipos, B., Biskup, S., Vladimirova, D., Sigle, S., Zsebedits, D., Fegeler, C., & Martens, U. M. (2022). ZvPM - Implementierung eines IT-gestützten überregionalen Molekularen Tumorboards (MTB). Oncology Research and Treatment, 45(suppl 2), 5–346. https://doi.org/https://doi.org/10.1159/000526456
Vladimirova, D., Bochum, S., Larribere, L., Kocher, S., Gallo, N., Sigle, S., Beutter, C. N. L., Biskup, S., Fegeler, C., & Martens, U. M. (2020). GASTRY: eine monozentrische Registerstudie zur Erfassung klinischer, molekularer und Lebensqualitätsparameter bei Patienten mit gastrointestinalen Tumoren / GASTRY: a monocentric registry study to record clinical, molecular and quality of life parameters in patients with gastrointestinal tumors. DGHO-Jahrestagung, Supplement Im Oncology Research and Treatment 2020. DGHO, Berlin. https://www.professionalabstracts.com/dgho2020/programme-dgho2020.pdf
Martens, U. M., Bochum, S., Mergel, F., Schiliro, A., Gallo, N., Pereira, P., Sipos, B., & Vladimirova, D. (2020). Rasches Therapieansprechen auf Larotrectinib bei mCRC-Patienten mit neuartiger onkogener NTRK-TP53 Fusion / Rapid clinical response to Larotrectinib in a mCRC patient with a novel oncogenic NTRK1-TP53 fusion. DGHO-Jahrestagung, Supplement Im Oncology Research and Treatment 2020, 133. https://www.professionalabstracts.com/dgho2020/programme-dgho2020.pdf
Bochum, S., Schall, L., Martens, U. M., Fegeler, C., & Pobiruchin, M. (2020). Pooling Clinical Cancer Registry Data with Public Genomic Datasets. Already an option to enhance real world evidence in precision oncology? DGHO-Jahrestagung, Supplement Im Oncology Research and Treatment 2020. DGHO. https://www.professionalabstracts.com/dgho2020/programme-dgho2020.pdf
Schall, L., Bochum, S., & Pobiruchin, M. (2020). Merging Genomics Public Datasets with Clinical Cancer Registry Data – Lessons Learned. GMDS.
Bochum, S. (n.d.). Platin-basierte Chemotherapeutika verlängern das Überleben von Pankreaskarzinom-Patienten mit einer Keimbahn-Mutation in BRCAness-Genen. Oncology Research and Treatment. https://doi.org/10.1159/000502425
Bochum, S., Vladimirova, D., Schiliro, A., Akkad, J., Autschbach, F., Finkeisen, D., Keil, A., Biskup, S., Fegeler, C., & Martens, U. M. (2019). Prolonged overall survival of pancreatic cancer patients with BRCAness germline mutations treated with DNA-damaging agents. Oncology Research and Treatment, 36. https://doi.org/10.1159/000502425
Bochum, S., & Martens, U. M. (2019). Keimbahnmutationen können über Therapieerfolg entscheiden. JATROS Hämatologie & Onkologie. https://www.universimed.com/at/article/onkologie/keimbahnmutationen-koennen-ueber-therapieerfolg-entscheiden-2122385
Berger, S., Bochum, S., Finkeisen, D., Schilliro, A., Autschbach, F., Bischof, M., Hagmueller, E., Pereira, P. L., Weickert, U., Sipos, B., Biskup, S., & Martens, U. M. (2018). Sustainable Response of a Patient With Metastasized Pancreatic Cancer and a Hypermutational Phenotype to Immunotherapy. New Therapeutic Concept for a Rare Subtype? JCO Precision Oncology, 2, 1–6. https://doi.org/10.1200/PO.18.00005
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Huang, C., Kratzer, M.-C., Wedlich, D., & Kashef, J. (2016). E-cadherin is required for cranial neural crest migration in Xenopus laevis. Developmental Biology, 411(2), 159–171. https://doi.org/10.1016/j.ydbio.2016.02.007
Becker, S. F., Langhe, R., Huang, C., Wedlich, D., & Kashef, J. (2012). Giving the right tug for migration: cadherins in tissue movements. Archives of Biochemistry and Biophysics, 524(1), 30–42. https://doi.org/10.1016/j.abb.2012.02.013
Schneider, M., Huang, C., Becker, S. F. S., Gradl, D., & Wedlich, D. (2014). Protocadherin PAPC is expressed in the CNC and can compensate for the loss of PCNS. Genesis (New York, N.Y.: 2000), 52(2), 120–126. https://doi.org/10.1002/dvg.22736
Gonnermann, C., Huang, C., Becker, S. F., Stamov, D. R., Wedlich, D., Kashef, J., & Franz, C. M. (2015). Quantitating membrane bleb stiffness using AFM force spectroscopy and an optical sideview setup. Integrative Biology: Quantitative Biosciences from Nano to Macro, 7(3), 356–363. https://doi.org/10.1039/c4ib00282b
Groh, N., Bühler, A., Huang, C., Li, K. W., van Nierop, P., Smit, A. B., Fändrich, M., Baumann, F., & David, D. C. (2017). Age-Dependent Protein Aggregation Initiates Amyloid-β Aggregation. Frontiers in Aging Neuroscience, 9, 138. https://doi.org/10.3389/fnagi.2017.00138
Groh, N., Gallotta, I., Lechler, M. C., Huang, C., Jung, R., & David, D. C. (2017). Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans. Journal of Visualized Experiments: JoVE, 129, 56464. https://doi.org/10.3791/56464
Huang, C., Wagner-Valladolid, S., Stephens, A. D., Jung, R., Poudel, C., Sinnige, T., Lechler, M. C., Schlörit, N., Lu, M., Laine, R. F., Michel, C. H., Vendruscolo, M., Kaminski, C. F., Kaminski Schierle, G. S., & David, D. C. (2019). Intrinsically aggregation-prone proteins form amyloid-like aggregates and contribute to tissue aging in Caenorhabditis elegans. ELife, 8, e43059. https://doi.org/10.7554/eLife.43059
Gallotta, I., Sandhu, A., Peters, M., Haslbeck, M., Jung, R., Agilkaya, S., Blersch, J. L., Rödelsperger, C., Röseler, W., Huang, C., Sommer, R. J., & David, D. C. (2020). Extracellular proteostasis prevents aggregation during pathogenic attack. Nature, 584(7821), 410–414. https://doi.org/10.1038/s41586-020-2461-z
Jechlinger, M., Pelz, R., Tretter, V., Klausberger, T., & Sieghart, W. (1998). Subunit composition and quantitative importance of hetero-oligomeric receptors: GABAA receptors containing alpha6 subunits. The Journal of Neuroscience: The Official Journal of the Society for Neuroscience, 18(7), 2449–2457. https://doi.org/10.1523/JNEUROSCI.18-07-02449.1998
Sieghart, W., Fuchs, K., Tretter, V., Ebert, V., Jechlinger, M., Höger, H., & Adamiker, D. (1999). Structure and subunit composition of GABA(A) receptors. Neurochemistry International, 34(5), 379–385. https://doi.org/10.1016/s0197-0186(99)00045-5
Felzmann, T., Buchberger, M., Jechlinger, M., Kircheis, R., Wagner, E., & Gadner, H. (2000). Xenogenization by tetanus toxoid loading into lymphoblastoid cell lines and primary human tumor cells mediated by polycations and liposomes. Cancer Letters, 161(2), 241–250. https://doi.org/10.1016/s0304-3835(00)00618-2
Janda, E., Lehmann, K., Killisch, I., Jechlinger, M., Herzig, M., Downward, J., Beug, H., & Grünert, S. (2002). Ras and TGF[beta] cooperatively regulate epithelial cell plasticity and metastasis: dissection of Ras signaling pathways. The Journal of Cell Biology, 156(2), 299–313. https://doi.org/10.1083/jcb.200109037
Jechlinger, M., Grünert, S., & Beug, H. (2002). Mechanisms in epithelial plasticity and metastasis: insights from 3D cultures and expression profiling. Journal of Mammary Gland Biology and Neoplasia, 7(4), 415–432. https://doi.org/10.1023/a:1024090116451
Grünert, S., Jechlinger, M., & Beug, H. (2003). Diverse cellular and molecular mechanisms contribute to epithelial plasticity and metastasis. Nature Reviews. Molecular Cell Biology, 4(8), 657–665. https://doi.org/10.1038/nrm1175
Jechlinger, M., Grunert, S., Tamir, I. H., Janda, E., Lüdemann, S., Waerner, T., Seither, P., Weith, A., Beug, H., & Kraut, N. (2003). Expression profiling of epithelial plasticity in tumor progression. Oncogene, 22(46), 7155–7169. https://doi.org/10.1038/sj.onc.1206887
Gotzmann, J., Fischer, A. N. M., Zojer, M., Mikula, M., Proell, V., Huber, H., Jechlinger, M., Waerner, T., Weith, A., Beug, H., & Mikulits, W. (2006). A crucial function of PDGF in TGF-beta-mediated cancer progression of hepatocytes. Oncogene, 25(22), 3170–3185. https://doi.org/10.1038/sj.onc.1209083
Jechlinger, M., Sommer, A., Moriggl, R., Seither, P., Kraut, N., Capodiecci, P., Donovan, M., Cordon-Cardo, C., Beug, H., & Grünert, S. (2006). Autocrine PDGFR signaling promotes mammary cancer metastasis. The Journal of Clinical Investigation, 116(6), 1561–1570. https://doi.org/10.1172/JCI24652
Waerner, T., Alacakaptan, M., Tamir, I., Oberauer, R., Gal, A., Brabletz, T., Schreiber, M., Jechlinger, M., & Beug, H. (2006). ILEI: a cytokine essential for EMT, tumor formation, and late events in metastasis in epithelial cells. Cancer Cell, 10(3), 227–239. https://doi.org/10.1016/j.ccr.2006.07.020
Siwko, S. K., Bu, W., Gutierrez, C., Lewis, B., Jechlinger, M., Schaffhausen, B., & Li, Y. (2008). Lentivirus-mediated oncogene introduction into mammary cells in vivo induces tumors. Neoplasia (New York, N.Y.), 10(7), 653–662, 1 p following 662. https://doi.org/10.1593/neo.08266
Podsypanina, K., Du, Y.-C. N., Jechlinger, M., Beverly, L. J., Hambardzumyan, D., & Varmus, H. (2008). Seeding and propagation of untransformed mouse mammary cells in the lung. Science (New York, N.Y.), 321(5897), 1841–1844. https://doi.org/10.1126/science.1161621
Jechlinger, M., Podsypanina, K., & Varmus, H. (2009). Regulation of transgenes in three-dimensional cultures of primary mouse mammary cells demonstrates oncogene dependence and identifies cells that survive deinduction. Genes & Development, 23(14), 1677–1688. https://doi.org/10.1101/gad.1801809
Rhim, A. D., Jechlinger, M., & Rustgi, A. K. (2015). Culturing mouse tumor cells. Cold Spring Harbor Protocols, 2015(6), 505–507. https://doi.org/10.1101/pdb.top069989
Rowald, K., Mantovan, M., Passos, J., Buccitelli, C., Mardin, B. R., Korbel, J. O., Jechlinger, M., & Sotillo, R. (2016). Negative Selection and Chromosome Instability Induced by Mad2 Overexpression Delay Breast Cancer but Facilitate Oncogene-Independent Outgrowth. Cell Reports, 15(12), 2679–2691. https://doi.org/10.1016/j.celrep.2016.05.048
Koehler, C., Sauter, P. F., Wawryszyn, M., Girona, G. E., Gupta, K., Landry, J. J. M., Fritz, M. H.-Y., Radic, K., Hoffmann, J.-E., Chen, Z. A., Zou, J., Tan, P. S., Galik, B., Junttila, S., Stolt-Bergner, P., Pruneri, G., Gyenesei, A., Schultz, C., Biskup, M. B., … Lemke, E. A. (2016). Genetic code expansion for multiprotein complex engineering. Nature Methods, 13(12), 997–1000. https://doi.org/10.1038/nmeth.4032
Weischenfeldt, J., Dubash, T., Drainas, A. P., Mardin, B. R., Chen, Y., Stütz, A. M., Waszak, S. M., Bosco, G., Halvorsen, A. R., Raeder, B., Efthymiopoulos, T., Erkek, S., Siegl, C., Brenner, H., Brustugun, O. T., Dieter, S. M., Northcott, P. A., Petersen, I., Pfister, S. M., … Korbel, J. O. (2017). Pan-cancer analysis of somatic copy-number alterations implicates IRS4 and IGF2 in enhancer hijacking. Nature Genetics, 49(1), 65–74. https://doi.org/10.1038/ng.3722
Havas, K. M., Milchevskaya, V., Radic, K., Alladin, A., Kafkia, E., Garcia, M., Stolte, J., Klaus, B., Rotmensz, N., Gibson, T. J., Burwinkel, B., Schneeweiss, A., Pruneri, G., Patil, K. R., Sotillo, R., & Jechlinger, M. (2017). Metabolic shifts in residual breast cancer drive tumor recurrence. The Journal of Clinical Investigation, 127(6), 2091–2105. https://doi.org/10.1172/JCI89914
Jechlinger, M., Kendrick, H., Smalley, M., & Vivanco, M. dM. (2017). See One, Do One, Teach One: A Practical Course on Methods in Mammary Gland Biology. Journal of Mammary Gland Biology and Neoplasia, 22(4), 215–219. https://doi.org/10.1007/s10911-017-9387-6
Alladin, A., Chaible, L., Garcia Del Valle, L., Sabine, R., Loeschinger, M., Wachsmuth, M., Hériché, J.-K., Tischer, C., & Jechlinger, M. (2020). Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation. ELife, 9, e54066. https://doi.org/10.7554/eLife.54066
Del Valle, L. G., Montero, M. G., & Jechlinger, M. (2022). Modification of Single Cells Within Mouse Mammary Gland Derived Acini via Viral Transduction. Methods in Molecular Biology (Clifton, N.J.), 2471, 185–194. https://doi.org/10.1007/978-1-0716-2193-6_9
D’Imprima, E., Garcia Montero, M., Gawrzak, S., Ronchi, P., Zagoriy, I., Schwab, Y., Jechlinger, M., & Mahamid, J. (2023). Light and electron microscopy continuum-resolution imaging of 3D cell cultures. Developmental Cell, 58(7), 616-632.e6. https://doi.org/10.1016/j.devcel.2023.03.001
van Amerongen, R., Bentires-Alj, M., van Boxtel, A. L., Clarke, R. B., Fre, S., Suarez, E. G., Iggo, R., Jechlinger, M., Jonkers, J., Mikkola, M. L., Koledova, Z. S., Sørlie, T., & Vivanco, M. dM. (2023). Imagine beyond: recent breakthroughs and next challenges in mammary gland biology and breast cancer research. Journal of Mammary Gland Biology and Neoplasia, 28(1), 17. https://doi.org/10.1007/s10911-023-09544-y
Yang, F., Bevilacqua, C., Hambura, S., Neves, A., Gopalan, A., Watanabe, K., Govendir, M., Bernabeu, M., Ellenberg, J., Diz-Muñoz, A., Köhler, S., Rapti, G., Jechlinger, M., & Prevedel, R. (2023). Pulsed stimulated Brillouin microscopy enables high-sensitivity mechanical imaging of live and fragile biological specimens. Nature Methods, 20(12), 1971–1979. https://doi.org/10.1038/s41592-023-02054-z
Yang, F., Bevilacqua, C., Hambura, S., Neves, A., Gopalan, A., Watanabe, K., Govendir, M., Bernabeu, M., Ellenberg, J., Diz-Muñoz, A., Köhler, S., Rapti, G., Jechlinger, M., & Prevedel, R. (2024). Author Correction: Pulsed stimulated Brillouin microscopy enables high-sensitivity mechanical imaging of live and fragile biological specimens. Nature Methods, 21(5), 922. https://doi.org/10.1038/s41592-024-02259-w
Starostecka, M., Jeong, H., Hasenfeld, P., Benito-Garagorri, E., Christiansen, T., Stober Brasseur, C., Gomes Queiroz, M., Garcia Montero, M., Jechlinger, M., & Korbel, J. O. (2025). Structural variant and nucleosome occupancy dynamics postchemotherapy in a HER2+ breast cancer organoid model. Proceedings of the National Academy of Sciences of the United States of America, 122(9), e2415475122. https://doi.org/10.1073/pnas.2415475122
Radic Shechter, K., Kafkia, E., Zirngibl, K., Gawrzak, S., Alladin, A., Machado, D., Lüchtenborg, C., Sévin, D. C., Brügger, B., Patil, K. R., & Jechlinger, M. (2021). Metabolic memory underlying minimal residual disease in breast cancer. Molecular Systems Biology, 17(10). https://doi.org/10.15252/msb.202010141
Vladimirova, D., Mbonu, P., Umapathy, M., Kothapalli, P., Otmacic, G., Akkad, J., Weyersberg, S., Martens, U. M., & Jechlinger, M. (2022). Etablierung einer Gewebe-Pipeline für multidimensionale Analysen beim metastasierten kolorektalen Karzinom. Oncology Research and Treatment, 45(suppl 2), 5–346. https://doi.org/https://doi.org/10.1159/000526456
Ozturk, M. S., Montero, M. G., Wang, L., Chaible, L. M., Jechlinger, M., & Prevedel, R. (2021). Intravital mesoscopic fluorescence molecular tomography allows non-invasive in vivo monitoring and quantification of breast cancer growth dynamics. Communications Biology, 4(1), 556. https://doi.org/10.1038/s42003-021-02063-8
Rubio, T., Weyershaeuser, J., Montero, M. G., Hoffmann, A., Lujan, P., Jechlinger, M., Sotillo, R., & Köhn, M. (2021). The phosphatase PRL-3 affects intestinal homeostasis by altering the crypt cell composition. Journal of Molecular Medicine, 99(10), 1413–1426. https://doi.org/10.1007/s00109-021-02097-9
Ronchi, P., Mizzon, G., Machado, P., D’Imprima, E., Best, B. T., Cassella, L., Schnorrenberg, S., Montero, M. G., Jechlinger, M., Ephrussi, A., Leptin, M., Mahamid, J., & Schwab, Y. (2021). High-precision targeting workflow for volume electron microscopy. Journal of Cell Biology, 220(9), e202104069. https://doi.org/10.1083/jcb.202104069
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Sigle, S., Werner, P., & Fegeler, C. (n.d.). Setting the Stage for Interoperable, Interinstitutional Application of (AI) Algorithms and Services for Radiology Use Cases: the OMI Protocol. 2. Heidelberger Frühjahrssymposium Medizinische Informatik. https://doi.org/https://doi.org/10.11588/heidok.00034810
Kaufmes, K., Klaumünzer, M., Vladimirova, D., Fegeler, C., & Sigle, S. (2024). Design and Implementation of Knowledge Artifacts to Support Molecular Tumorboard Preparation Processes. Gesundheit – Gemeinsam. Kooperationstagung Der Deutschen Gesellschaft Für Medizinische Informatik, Biometrie Und Epidemiologie (GMDS), Deutschen Gesellschaft Für Sozialmedizin Und Prävention (DGSMP), Deutschen Gesellschaft Für Epidemiologie (DGEpi), Deutschen Gesellschaft Für Medizinische Soziologie (DGMS) Und Der Deutschen Gesellschaft Für Public Health (DGPH). https://doi.org/10.3205/24GMDS053
Mathes, G., Bochum, S., Werner, P., Fegeler, C., & Sigle, S. (2024). Design and Implementation of a Community Driven Clinical Trial Information Management System for Precision Oncology Settings. In R. Röhrig, N. Grabe, U. H. Hübner, K. Jung, U. Sax, C. O. Schmidt, M. Sedlmayr, & A. Zapf (Eds.), Studies in Health Technology and Informatics. IOS Press. https://doi.org/10.3233/SHTI240844
Vishnevskaya, V., Sigle, S., & Fegeler, C. (2024). From Data Mining of Public Sources to Clinical Decision Support in Personalized Medicine. In J. Mantas, A. Hasman, G. Demiris, K. Saranto, M. Marschollek, T. N. Arvanitis, I. Ognjanović, A. Benis, P. Gallos, E. Zoulias, & E. Andrikopoulou (Eds.), Studies in Health Technology and Informatics. IOS Press. https://doi.org/10.3233/SHTI240734
Beutter, C. N. L., Berger, S., Martens, U. M., & Fegeler, C. (2024). Report from Health Professionals and Cancer Patients: Experiences and Wishes from Clinical Practice About the Integration of Quality of Life with a Health App. In J. Mantas, A. Hasman, G. Demiris, K. Saranto, M. Marschollek, T. N. Arvanitis, I. Ognjanović, A. Benis, P. Gallos, E. Zoulias, & E. Andrikopoulou (Eds.), Studies in Health Technology and Informatics. IOS Press. https://doi.org/10.3233/SHTI240373
Beutter, C. N. L., Zeller, K., & Fegeler, C. (2023). Lessons learned of a patient-centered development of an app for measuring Quality of Life in oncology. https://doi.org/10.3205/23GMDS064
Beutter, C. N. L., Block, N., Berger, S., Edo-Ferrando, P., Heinz, B., Läufer, K., Lang, B., Mächtlen, K., Münkel, S., Rannert, D., Zwerenz-Kopp, F., & Fegeler, C. (2024). Lebensqualität bei Krebserkrankungen – Integration in die Versorgung (working paper von Versorgern und Betroffenen). https://doi.org/10.34657/13451
Sigle, S., Werner, P., Schweizer, S., Caldeira, L., Hosch, R., Dyrba, M., & Fegeler, C. (2024). Bridging the Gap Between (AI-) Services and Their Application in Research and Clinical Settings Through Interoperability: the OMI-Protocol. https://doi.org/10.34657/13458
Lingg, M., Beutter, C., Sigle, S., Zsebedits, D., & Fegeler, C. (2023). Challenge of Detecting Personal Deviations and Trends in Sensor Based Activity Data. In R. Röhrig, N. Grabe, M. Haag, U. Hübner, U. Sax, C. Oliver Schmidt, M. Sedlmayr, & A. Zapf (Eds.), Studies in Health Technology and Informatics. IOS Press. https://doi.org/10.3233/SHTI230711
Beutter, C. N. L., Werner, P., Sigle, S., Martens, U. M., & Fegeler, C. (2023). Assessing Quality of Life Using FHIR – How to Combine Patient Reported Outcome with Patient Generated Data for Better Compliance. In M. Hägglund, M. Blusi, S. Bonacina, L. Nilsson, I. Cort Madsen, S. Pelayo, A. Moen, A. Benis, L. Lindsköld, & P. Gallos (Eds.), Studies in Health Technology and Informatics. IOS Press. https://doi.org/10.3233/SHTI230083
Beutter, C. N. L., Vladimirova, D., Martens, U. M., & Fegeler, C. (2023). Clinical use of quality of life data for treatment and survivorship in oncology. Journal of Clinical Oncology, 41(16_suppl), e24044–e24044. https://doi.org/10.1200/JCO.2023.41.16_suppl.e24044
Sigle, S., Werner, P., & Fegeler, C. (2022). Implementation of an Interoperable Order-Entry Process for Virtual Molecular Tumorboards. Oncology Research and Treatment, 45(suppl 2), 5–346. https://doi.org/https://doi.org/10.1159/000526456
Beutter, C. N. L., Imeraj, E., Martens, U. M., & Fegeler, C. (2022). Concept of a data driven system to accomplish a longitudinal survey of quality of life in oncology. https://doi.org/10.3205/22GMDS030
Mathes, G., Sigle, S., & Fegeler, C. (2022). Design and implementation of a decision support system for eligibility of patients within oncologic precision trials. https://doi.org/10.3205/22GMDS003
Beutter, C. N. L., Martens, U. M., & Fegeler, C. (2022). From patient generated data to precision medicine: An example of a patient-centered application in oncology. Journal of Clinical Oncology, 40(16_suppl), e13559–e13559. https://doi.org/10.1200/JCO.2022.40.16_suppl.e13559
Sigle, S., Kaufmes, K., Werner, P., Bochum, S., Martens, U., & Fegeler, C. (2021). Variant Browser: A comprehensive information retrieval tool for molecular genetics in a precision oncology setting. Oncology Research and Treatment, 44(2), 96–97. https://doi.org/10.1159/000518417
Sigle, S., Kaufmes, K., Werner, P., Bochum, S., Martens, U., & Fegeler, C. (2021). Introducing Variant Browser – a Meta Search Plattform for Genetic Variants. DocAbstr. 48. https://doi.org/10.3205/21gmds054
Sigle, S., Bochum, S., Werner, P., Walter, D., Martens, U. M., & Fegeler, C. (2020). Taming Data Sources to enable interoperable Clinical Decision Support for Virtual Molecular Tumor Boards. DGHO-Jahrestagung, Supplement Im Oncology Research and Treatment 2020, 69. https://doi.org/10.1159/isbn.978-3-318-06715-6
Sigle, S. (2020, November 17). Establishing Clinical Decision Support for Virtual Molecular Tumor Boards. FHIR Dev Days 2020. https://www.devdays.com/wp-content/uploads/2020/12/zibbw_201117_StefanSigle_Establishing_Clinical_Decision_Support_for_Virtual_Molecular_Tumor_Boards.pdf
Sigle, S., Werner, P., Bochum, S., Finkeisen, D., Martens, U. M., & Fegeler, C. (2020). Interoperable Knowledge- and Decision Support Components in Virtual Molecular Tumor Boards. GMDS.
Molekularpathologie on FHIR: Der Clinical Genomics Implementation Guide. (2020). DMEA – SParks.
Keil, A., Sigle, S., Bochum, S., & Fegeler, C. (2019). Drowning in Information: The lack of integrating Knowledge Databases. Oncology Research and Treatment, 61. https://doi.org/10.1159/000502425
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